Just after midnight on Thursday, a stolen car allegedly failed to stop for police in Hoppers Crossing, Wyndham.
The Air Wing tracked the car to Laverton before police successfully deployed stop sticks on Old Geelong Road.
Five children aged between 15 and 17 were arrested and charged with the theft of a motor vehicle, theft from a motor vehicle and handling stolen goods.
A further 26 people were caught up with during the Wednesday night operation.
A 29-year-old man was arrested in Point Cook in connection to the alleged theft of a car in Werribee in February.
A 24-year-old motorbike rider allegedly failed to stop for police on Palmers Road in Williams Landing before running a red light about 9.45pm.
Air Wing followed from above, observing the rider allegedly reach speeds of 170km/h, before officers moved in and arrested him out of the front of his Tarneit address.
The man had his bike impounded and is expected to be charged on summons with driving unregistered, driving unlicensed, disobeying a red light and exceeding the speed limit.
A 31-year-old motorbike rider who was observed allegedly travelling at speed with no headlights on in Hoppers Crossing about 10pm.
The Air Wing followed from above before officers on the ground arrested him at his Hoppers Crossing address.
The man had his bike impounded and is expected to be charged on summons with driving unregistered, driving with no headlights and exceeding the speed limit.
An 18-year-old New South Wales man was caught allegedly travelling around 30km over the speed limit in Werribee around 10pm.
He had his vehicle impounded and is expected to be charged on summons with driving unlicensed, driving unregistered and speeding.
A total of 21 penalty notices were issued for drink driving, driving unregistered, speeding, and for a learner driver failing to have an accompanying driver.
During the operation, more than 192 vehicles were checked and 41 were intercepted.
A total of five vehicles were impounded.